ABI PRISM® 310 Genetic Analyzer

A Benchmark in Laboratory Automation Molecular biology laboratories need technology that is easy to use yet
sufficiently flexible for a variety of applications. To meet this need, Applied Biosystems built upon its
proven multicolor fluorescent labeling technology to achieve a completely automated, yet flexible system to
sequence, size, and quantitate nucleic acids. This system is the ABI PRISM® 310 Genetic Analyzer.
The 310 Genetic Analyzer is the standard in laboratory convenience. After samples are placed in the
sample tray, virtually all aspects of analysis are automated. There is no need to pour conventional
polyacrylamide and agarose slab gels or to load samples for electrophoresis. Instead, every function—from sample and
gel loading to data collection and analysis is computer controlled. Equally important, preformulated
ABI PRISM® polymers eliminate the guesswork involved with preparing gel materials. The convenience of
the system is further enhanced by ABI PRISM® reagent kits for DNA sequencing or PCR product analysis
and by access to Applied Biosystems worldwide technical support.

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Description

Versatile, Affordable, Easy-to-Use, and Almost Hands-Free

The 310 Genetic Analyzer – the ideal alternative to gel-based DNA analysis for any research, clinical, forensic lab, or schools for teaching purposes – enables you to automate applications from de novo sequencing to SNP validation and linkage mapping. This versatile analyzer is so easy to use, that once it is installed and you have completed our short training course, all you have to do is load your samples, set up the Data Collection software and click the Start button.

Integrated Data Analysis Tools Reduce Time-to-Results

The 310 Genetic Analyzer software suite allows you to generate more meaningful data with less work.

The software suite includes (for U.S. customers, this suite also includes tools that assist with FDA 21CFRpart 11 compliance requirements):

  • Data Collection Software (supplied with the instrument) – Manages your instrument setup, controls instrument operations, allows real-time data visualization, and performs diagnostics. Features include auto-analysis with Sequencing Analysis Software, flexibility to use any choice in dye set option, and optimized sequencing run modules that enable you to perform many different applications.
  • Sequencing Analysis Software – Performs basecalling; assigns quality values; trims, displays, edits, and prints DNA sequencing data using the KB™ basecaller software.
  • Seqscape® Software – A software program specifically designed to support re-sequencing projects data analysis.
  • GeneMapper® Software – Enables configurable, automated allele calling. The closely related GeneMapper®ID Software is specifically designed for human-identity applications.
  • MicroSeq® Software – Enables you to compare the sequence of an unidentified bacteria or fungi to a validated library for positive identification and taxonomic classification.

Extensive, Experienced Support

Solutions offered to you by the 310 Genetic Analyzer extend beyond just technologies and chemistry. We also have the industry’s most experienced and knowledgeable team of support specialists to help you get the most out of your instruments, reagents, and research.

Applied-Biosystems Sequencing Instruments

Easy Instrument Set-up Simply install the capillary, load the gel pump with polymer, place your samples in the autosampler tray, and instrument set-up is complete. Automated Gel Loading The 310 system gel pump automatically fills the capillary with either DNA sequencing or fragment analysis polymer. Switching from DNA sequencing to PCR product analysis is as easy as installing a different capillary and polymer. Automated Sample Loading The autosampler, using an electrokinetic injection, sequentially loads each sample into the capillary for electrophoresis. ABI PRISM® Separation Polymers The 310 Genetic Analyzer separates DNA fragments by using POP™ Performance Optimized Polymers, which yield extremely reproducible results. The POP™ polymer family includes POP-6™ polymer for high-resolution applications such as DNA sequencing, and POP-4™ polymer, which provides faster runs for applications such as microsatellite analysis, AFLP® Kit, and rapid sequencing. We also offer a general purpose polymer that is user-modifiable for a variety of genotyping applications. This polymer allows you to alter polymer concentration or add reagents such as urea or glycerol. Multiple Dye Detection The 310 Genetic Analyzer simultaneously detects up to five different fluorescent dyes in a single capillary providing multicolor analysis. This process is further simplified by fluorescent terminator sequencing chemistry, which allows you to perform single tube sequencing reactions. For genotyping, multicolor detection increases throughput by allowing you to multiplex several PCR samples labeled with multiple dyes. A labeled internal standard can be added to each run to provide exceptional fragment sizing reproducibility. ABI PRISM® System Integration The 310 Genetic Analyzer is fully integrated with proven ABI PRISM® reagents and software for DNA sequencing and genotyping applications. The ABI PRISM® 310 Analyzer Windows®-compatible system allows data to be easily transferred between different platforms such as the ABI PRISM® 3100 and 3100-Avant Genetic Analyzers as well as to downstream analysis software, the industry standards for automated genotyping, sequencing, and mutation analysis.

Capacity: 48 or 96 sample tubes
Capillary Length: 47 cm, 61 cm
Compatible Polymers: POP™ Conformational Analysis Polymer, POP-4™, POP-6™
Dimensions: Width 60.96 cm (24 in) x Depth 55.88 cm (22 in) x Height 86.36 cm (34 in)
External Computer: 512 MB RAM, 80 GB Hard Drive, DVD-ROM RW 16/16/4X, Dell® GX-280 with Pentium 4 (2.8 GHz)
For Use With (Equipment): 310 Genetic Analyzer
Format: Tube(s)
Laser Features: Dye excitation by 488- and 514-nm lines of the 10-mW argon ion laser
Number of Capillaries: 1 Capillary
Operating Environment: Humidity < 80%, Temperature: 15°C-30°C
Operating System: Windows XP
Performance: Single base detection up to 250 bases with 0.15 Standard deviation
Plan Duration: 1 Year Warranty
Plate Handling: Holds 48- or 96- sample tubes for unattended operation
Polymer Consumption: Stablity on instrument 7 days, Up to 200 sample runs per 3 ml polymer
Product Size: 1 instrument
Read Length: Standard Mode – 600 Basecalls per run
System Requirements: PC: 512 Mb RAM; Pentium 4 (2.8 GHz); 80 GB hard drive
Throughput: Standard Mode – 5220 basecalls/24 hr (9 runs x 580 basecalls per run)
Voltage Limit: 15 kV
Weight: 94.5 kg (208.34 lbs)

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