Applied Biosystems®7500 Fast Dx Real-Time PCR Instrument, with laptop computer

The Applied Biosystems® 7500 Fast Dx Real-Time PCR Instrument with SDS Software is a real-time nucleic acid amplification and five-color fluorescence detection system available for in vitro diagnostic use. The 7500 Fast Dx Real-Time PCR Instrument delivers the performance required for high-quality results in a 96-well format.

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Description

Convenient 96-well Format

The 7500 Fast Dx Real-Time PCR Instrument minimizes the effort required to perform sample setup while also allowing the use of tube strips.

Intuitive Software to Run Pre-optimized Protocols

The 7500 Fast Dx instrument’s Sequence Detection Software Security, Auditing, and E-Signature module offers the flexibility to develop assays and also run pre-optimized protocols for users operating in a secure environment.

Instrument Service and Support

The 7500 Fast Dx Real-Time PCR Instrument is supported by our AB Assurance Service Plan for Diagnostics, which includes:

  • Instrument Installation Qualification, Operation Qualification, and Performance Qualification (IQ⁄OQ⁄PQ)
  • Spectral calibration and software setup
  • Emergency repair, including parts and labor, and OQ⁄PQ after any major part change
  • Planned maintenance every 12 months

Europe:

For in vitro Diagnostic Use. The 7500 Fast Dx Real-Time PCR Instrument meets the requirements of the in vitro Diagnostic Medical Devices Directive (98⁄79⁄EC). The CE IVD-registered 7500 Fast Dx Real-Time PCR Instrument is for distribution and use in specific European countries only.

USA:

For in vitro Diagnostic Use. The 7500 Fast Dx Real-Time PCR instrument is 510(k)-cleared (K082562). The customer is responsible for any validation of assays and compliance with any regulatory requirements that pertain to their procedures and instrument use.

Applied-Biosystems Realtime PCR Instruments

  • 96-well format eases plate setup if automation is not available
  • Accommodates tube strips, which can be capped immediately after pipetting each sample
  • Fast mode completes runs in less than forty minutes
  • Optional standard mode facilitates use of standard length real-time PCR assays without changing thermal cycling parameters
  • 5-color variable excitation enables multiplex assays

Calibrated Dye: Cy3, Cy5, FAM, JOE, NED, ROX, SYBR Green, TAMRA, Texas Red, VIC
Capacity: 1 x 96-well plate (Fast), 12 x 8-tube strips
Dimensions: 34 cm (13.39 in) (W) x 49 cm (19.29 in) (H) x 45 cm (17.72 in) (D)
Dynamic Range: 9 logs of linear dynamic range
External Computer: Laptop computer provided
For Use With (Equipment): 7500 Fast Dx System
Format: 8-well strips, 96-well plate
Optics: CCD Camera, Five excitation and five emission filters, Halogen light source
Passive Reference Dye: Other Passive Reference Dye, ROX (Pre-mixed), ROX (Separate Tube)
Peak Block Ramp Rate: 5.5°C⁄sec
Precision: Distinguish between 5,000–10,000 genome equivalents (two-fold copy number difference) with 99.7% confidence
Product Size: 1 system
Reaction Speed: Fast, Standard
Reaction Volume Range: 10-30 µl
Run Time: <2 hrs (Standard), 36 min (Fast)
Sample Ramp Rate: Fast mode: ± 3.5°C⁄sec, Standard mode: ± 1.6°C⁄sec
Sensitivity: Down to 1 copy of human RNAse P gene
Temperature Accuracy: ±0.5°C
Temperature Range (Metric): 4.0-99.9 °C
Temperature Uniformity: ±1°C
Thermal Cycling System: Peltier-Based System
Throughput: 4 to 5 96-well plates per day
Weight: 34 kg (75 lb)

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